BRAF Pyrosequencing Analysis Aided by a Lookup Table

BRAF pyrosequencing analysis aided by a lookup table.

OBJECTIVES BRAF mutations have substantial therapeutic, diagnostic, and prognostic significance, so detecting and specifying them is an important part of the workload of molecular pathology laboratories. Pyrosequencing assays are well suited for this analysis but can produce complex results. Therefore, we introduce a pyrosequencing lookup table based on Pyromaker that assists the user in genera...

Revisiting a Masked Lookup-Table Compression Scheme

Computing Mod with a Variable Lookup Table

Encryption algorithms are designed to be difficult to break without knowledge of the secrets or keys. To achieve this, the algorithms require the keys to be large, with some having a recommend size of 2048bits or more. However most modern processors only support computation on 64-bits at a time. Therefore standard operations with large numbers are more complicated to implement. One operation th...

Accurate Function Approximations by Symmetric Table Lookup and Addition

This paper presents a high-speed method for accurate function approximations. This method employs parallel table lookups followed by multi-operand addition. It takes advantage of leading zeros and symmetry in the table entries to reduce the table sizes. By increasing the number of tables and the number of operands in the multi-operand addition, the amount of memory is signiicantly reduced. This...

Pyrosequencing data analysis software: a useful tool for EGFR, KRAS, and BRAF mutation analysis

BACKGROUND Pyrosequencing is a new technology and can be used for mutation tests. However, its data analysis is a manual process and involves sophisticated algorithms. During this process, human errors may occur. A better way of analyzing pyrosequencing data is needed in clinical diagnostic laboratory. Computer software is potentially useful for pyrosequencing data analysis. We have developed s...